Spotlight on Science Meetings, Conferences and Events brings you information on the following event: Genetic Code Expansion 2017 Workshop
This intensive laboratory and lecture course will provide participants with the theoretical and practical knowledge to utilize existing and emerging genetic code expansion technology. The laboratory component will focus on troubleshooting GCE tools with attendees and their genes in E. coli expression systems. The workshop will have a demonstration section on expression unnatural protein in mammalian systems with standard controls.
The laboratory component will focus on two common needs in the genetic code expansion field: 1) incorporation of an existing non-canonical amino acid into protein of interest 2) screening a new non-canonical amino acid against a panel of synthetases to determine if the desired non-canonical amino acid can be incorporated with current genetic code expansion tools. As part of the laboratory component of the course, participants will be given the opportunity to bring their research gene of interest and/or their non-canonical amino acid to the workshop to test their expression and incorporation along with optimized controls.
The lecture component will focus on how new genetic code expansion components are generated and attributes of orthogonality, efficiency, fidelity and permissivity are defined, measured and are conditionally dependent. The major applications now afforded by the new non-canonical amino acids will be discussed with a focus on the future of each field and its limitations.