Course - Cold Spring Harbor, NY, United States
The Eukaryotic Gene Expression Course is designed for students, postdocs, and principal investigators who have recently ventured into the exciting area of gene regulation.The course will focus on state-of-the-art strategies and techniques employed in the field. Emphasis will be placed both on in vitro and in vivo protein-DNA interactions and on novel methodologies to study gene regulation. Students will make nuclear extracts, perform in vitro transcription reactions and measure RNA levels using primer extension. Students will isolate transcription factor complexes and assess their activity in functional assays. In addition, students will learn techniques for the assembly and analysis of chromatin in vitro. This will include transcription assays, chromatin footprinting and chromatin remodeling assays.
Organization: Cold Spring Harbor Laboratory
Cold Spring Harbor, United States
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