Course - Heidelberg, Germany
The analysis of transcripts in minute samples is often used to increase the knowledge in a plethora of research fields. Precise sampling is crucial for the success of the approach and depends on the nature of the sample and the goal of the experiment. This course will present flow cytometry, Fluidigm and laser capture microdissection as methods to obtain single cells for the subsequent gene expression analysis, which cannot be performed by conventional RNA methods due to their low sensitivity. This course will present different techniques to assess gene expression in individual cells, including RT-qPCR, Next Generation Sequencing and Proximity Extension Assay. Data collected by these methods will be analysed in the very crucial session of data analysis, which constitutes another strong component of the course.
- Organization: CCO EMBL
Fri, Apr 17, 2015 - Wed, Apr 22, 2015
Advanced Training Centre
Heidelberg, Germany
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