Course - Cold Spring Harbor, NY, United States
The Eukaryotic Gene Expression Course is designed for students, postdocs, and principal investigators who have recently ventured into the exciting area of gene regulation. The course will focus on state-of-the-art strategies and techniques employed in the field. Emphasis will be placed both on in vitro and in vivo protein-DNA interactions and on novel methodologies to study gene regulation. Students will make nuclear extracts, perform in vitro transcription reactions and measure RNA levels using primer extension. Students will isolate transcription factor complexes and assess their activity in functional assays. In addition, students will learn techniques for the assembly and analysis of chromatin in vitro. This will include transcription assays, chromatin footprinting and chromatin remodeling assays.
Experience with basic recombinant DNA techniques is a prerequisite for admission to this course. Lectures by the instructors will cover the current status of the gene expression field, theoretical aspects of the methodology, and broader issues regarding strategies for investigating the regulation of gene expression in eukaryotes. Emphasis will be placed on advantages and limitations of specific techniques, and data interpretation. The students are encouraged and expected to actively participate in these discussions.
(Courtesy of Eukaryote Gene Expression, via en.wikipedia.org)
Tue, Jul 23, 2013 - Mon, Aug 12, 2013
CSHL Campus, Cold Spring Harbor, United States
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