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Course - Bergisch Gladbach/Cologne, Germany

 

T cells play an essential role in the immune system, as they are responsible for initiation and regulation of immune responses to pathogens. T cells are a heterogeneous cell population, comprising a multitude of subpopulations with distinct functions and phenotypes. Flow cytometry is a widely used method for cell analysis and is often used to elucidate the functions and roles of T cells in health and disease. However, the detection of antigen-specific T cells, for example, is hampered by their very low frequency.

This course provides hands-on experience for detecting and analyzing antigen-specific and non-specific human T cell subsets. Participants will be introduced to immunomagnetic pre-enrichment as a simple method enabling the reliable detection and analysis of rare cell types. By combining this method with intracellular cytokine staining, antigen-specific T cell subsets can be analyzed in detail. Furthermore, this course conveys both theoretical aspects and extensive practical experience of multicolor analysis of numerous T cell subsets from human blood.
The training covers all subjects from a profound introduction via hands-on training through to comprehensive troubleshooting.

Organization: Miltenyi Biotec

T cell flow cytometry – analyzing antigen-specific T cells extra- and intracellulary