Course - Cold Spring Harbor, NY, United States
Xenopus is an extraordinary in vivo model for cell and developmental biology. The ease of loss- and gain-of-function approaches allows rapid mechanistic analysis; this can be combined with classic embryological manipulations and state of the art imaging. As advances in human genomics rapidly expand our list of novel disease genes, Xenopus is emerging as a powerful, high-throughput system for studying unknown gene function.
Techniques covered in this course will include microinjection, molecular manipulations such as CRISPR knockouts, transgenics, and mRNA overexpression. These can be combined with explant and transplant methods to simplify or test tissue level interactions. To visualize subcellular and intercellular activities, we will introduce a variety of imaging methods including time-lapse, fluorescent and confocal microscopy. Additional methods include mRNA in situ hybridization and protein immunohistochemistry as well as basic bioinformatic techniques for gene comparison and functional analysis.
- Organization: Cold Spring Harbor Laboratory
Thu, Apr 09, 2015 - Tue, Apr 21, 2015
CSHL Campus
Cold Spring Harbor, United States
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