Course - Cold Spring Harbor, NY, United States
The Advanced Bacterial Genetics course presents logic and methods used in the genetic dissection of complex biological processes in diverse bacteria. Laboratory methods include: classical mutagenesis using transposons, mutator strains, and chemical mutagens; recombineering with single and double stranded DNA; detection of gene expression changes using various reporter genes; the mapping of mutations using genetic and physical techniques; modern approaches to the generation and analysis of targeted gene disruptions and fusions using PCR and cloning methods; epitope insertion mutagenesis; site-directed mutagenesis; and fluorescence microscopy. Key components of the course will be the use of sophisticated genetic methods in the analysis of model bacteria (including Escherichia coli, Salmonella, Bacillus subtilis, and Vibrio cholerae), and the use of the wealth of new genomic sequence information to motivate these methods.
Organization: Cold Spring Harbor Laboratory
Wed, Jun 03, 2015 - Tue, Jun 23, 2015
CSHL Campus
Cold Spring Harbor, United States
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