X-Ray Methods in Structural Biology
Course - Cold Spring Harbor, NY, United States
Crystallography and X-ray diffraction yield a wealth of structural information unobtainable through other methods. This intensified laboratory/computational course focuses on the major techniques used to determine the three-dimensional structures of macromolecules. It is designed for scientists with a working knowledge of protein structure and function, but who are new to macromolecular crystallography. Topics to be covered include basic diffraction theory, crystallization (proteins, nucleic acids and complexes), crystal characterization, X-ray sources and optics, synchrotrons, crystal freezing, data collection, data reduction, multiple isomorphous replacement, multiwavelength anomalous diffraction, molecular replacement, solvent flattening, non-crystallographic symmetry averaging, electron density interpretation, molecular graphics, structure refinement, structure validation, coordinate deposition and structure presentation. Participants learn through extensive hands-on experiments. One or more proteins are crystallized and the structure(s) determined by several methods, in parallel with lectures on the theory and informal discussions behind the techniques. Applicants should be familiar with the creation and editing of simple text files on Linux workstations using a screen based editor (either vi or emacs).
(Courtesy of X-ray crystallography, via london-nano.com)
For more information, pease visit: http://www.cshl.edu/meetings
October 14, 2013 - October 29, 2013
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, NY 11724
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