Course - Cologne, Germany
This 3.5-day training course focuses on improving ES/iPS generation, cell culture and characterization. The course covers all critical steps required for ES/iPS research. Encompassing theoretical presentations and practical lab sessions, participants will learn advanced ways of cell preparation, cell selection with magnetic cell separation and basic cell analysis with flow cytometry.
Course lectures and exercises include:
Reprogramming techniques for the generation of iPS cell lines
Characterization of pluripotent stem cells with different methods
Cultivation strategies of pluripotent stem cells
Pluripotent stem cell differentiation
Basics of magnetic cell separation for pluripotent stem cell cultures
Basics of flow cytometry
Discussion and trouble-shooting with experienced users from R&D
Practical laboratory parts include:
Setup and maintenance of iPS cell cultures
Transfection procedure for mRNA-based reprogramming
Hands-on introduction to flow cytometry
iPS cell characterization with different methods, including flow cytometry
Magnetic separation of differentiated from undifferentiated iPS cells
Removal of feeder cells with magnetic separation
Improved dissociation of murine embryoid bodies
(Courtesy of Human Embryonic Stem Cells, via sciencedaily.com)
Tue, Jul 02, 2013 - Fri, Jul 05, 2013
MACS Academy, Miltenyi Biotec GmbH, Friedrich-Ebert-Str. 68,
Bergisch Gladbach/Cologne, Germany
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