Advanced Techniques in Molecular Neuroscience
Course - Cold Spring Harbor, United States
This laboratory and lecture course is designed to provide neuroscientists at all levels with a conceptual and practical understanding of several of the most advanced techniques in molecular neuroscience. The course curriculum is divided into three sections: an extensive and up-to-date set of laboratory exercises, daily lectures covering the theoretical and practical aspects of the various methods used in the laboratory, and a series of evening research seminars. The informal and interactive evening lectures will be given by leading molecular neuroscientists and will serve to illustrate the ways in which the various experimental approaches have been used to advance specific areas of neurobiology. In this year's course, the laboratory portion will include topics such as: an introduction to the design and use of animal virus vectors in neurobiology; the use of small interfering RNAs (siRNA) for regulating the expression of specific genes in neurons; practical exercises in gene delivery systems including mammalian cell transfection protocols and single cell electroporation techniques for targeted gene transfer in vivo; an introduction to overall strategies, use and design of BAC transgenic vectors; quantitative real time RT-PCR analyses from small numbers of cells (RNA purification, PCR optimization, interpretation of results); and application of RNA amplification (aRNA) for single cell analysis. Each laboratory module will be followed by presentations and discussions focused on data interpretation, protocol troubleshooting, and ways to modify and potentially improve existing techniques.
(Courtesy of Cold Spring Harbor, US, via labdesignnews.com)
Advanced Techniques in Molecular Neuroscience
Tue, Jul 02, 2013 - Thu, Jul 18, 2013
CSHL Campus, Cold Spring Harbor, United States
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