EMBO Practical Course: 'Imaging of Neural Development in Zebrafish'
Course - Karlsruhe, Germany
A fundamental question in developmental biology is how myriads of neurons get specified from progenitor cells and become organized into complex functional networks. Little is known on the dynamics of neural lineage decisions, cell polarity and migratory events during nervous system patterning and morphogenesis.
The objective of the EMBO Practical Course is to provide theoretical and practical background on the zebrafish model system for direct assessment of open questions using novel microscopy techniques
List of technologies and model paradigms that the applicants will get experience on:
Optogenetics
Live imaging of synapse formation
Functional imaging of genetically encoded calcium indicatorsIn vivo analysis of interactions between microglia and neurons
Cell lineage labelling and cell ablation in the developing retina
Electroporation and retrograde tracing
Spreading of morphogens within the neural tube
Asymmetric cell divisions in the spinal cord
Cell tracking using photoconvertible fluorophores
Optokinetic response
Differentiation of zebrafish neural stem cells in vitro
Digital Light Sheet Microscopy (DSLM)
Fluorescent Correlation Spectroscopy (FCS)
(Courtesy of Karlsruhe Institute of Technology, via http://events.embo.org/13-neural-zebrafish/)
EMBO Practical Course: 'Imaging of Neural Development in Zebrafish'
Sun, Sep 08, 2013 - Sun, Sep 15, 2013
Karlsruhe Institute of Technology, Karlsruhe, Germany
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