Spotlight on Science Meetings, Conferences and Events brings you information on the following event: The Aurion Immuno Gold Silver Staining Workshop
The objective of the course is to provide researchers with the opportunity to learn the theory and practice of Immuno Gold labeling. Participants will process their own samples under the expert guidance of our tutors, who are experts in Immuno Gold Silver Staining techniques.
During the workshop attendees will receive theory, including but not limited to immune detection, in situ hybridization, silver enhancement, as well as background issues. There will be time for practice, as well. Attendees will be able to work with their own specimens, as well as ones we will have prepared. A full review of incubation methods, testing of antigenicity and reactivity, complete principles of Immuno Gold labeling, and preparation of conjugates for EM and LM will be covered.
Main Curriculum
- The properties of gold particles and their protein conjugates
- Theory underlying Immuno Gold labeling protocols
- Silver enhancement of gold particles
- Immuno Gold labeling on a variety of sample preparations of OLM
- Immuno Gold labeling for EM
- Pre-embedment Immuno Gold labeling using ultra small gold conjugates and silver enhancement
- Post-embedment Immuno Gold labeling on plastic and ToKuyasu cryo sections using conventional colloidal conjugates and ultra small gold conjugates
- Manual and automated Immuno Gold labeling
- Pre/Post-embedment double Immuno Gold labeling
- Background minimization in Immuno Gold labeling
- Troubleshooting
The EMS Microscopy Academy
Hatfield, PA
Phone: 215-412-8400
stacie@ems-secure.com
http://www.emsdiasum.com/microscopy/academy/courses/immunogold.aspx
Wednesday, May 10, 2017 to Friday, May 12, 2017
