Course - Cold Spring Harbor, NY, United States
Recent advances in the generation and selection of antibodies from combinatorial libraries allow for the rapid production of antibodies from immune and non-immune sources. This intensive laboratory/lecture course will focus on the construction of combinatorial antibody libraries expressed on the surface of phage and selection of desired antibodies from the library. Students will learn the theoretical and practical aspects of constructing combinatorial libraries from immune and non-immune sources as well as the construction of synthetic antibody libraries. Antibodies will be selected from the library by panning. Production, purification and characterization of Fab fragments expressed in E.coli will also be covered. Epitopes will be selected from peptide libraries and characterized.
- Organization: Cold Spring Harbor Laboratory
- For more information, please visit: http://meetings.cshl.edu/courses/2014/c-mac14.shtml
Wed, Nov 05, 2014 - Tue, Nov 18, 2014
Cold Spring Harbor Laboratory
Cold Spring Harbor, NY, United States
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