Course - Cold Spring Harbor, NY, United States
This course will focus on advanced quantitative fluorescence microscopy techniques used for imaging a range of biological specimens, from cells to single molecules. The course is designed for cell and molecular biologists with little or no microscopy experience who wish to begin utilizing microscopy in their own research. Students will gain a theoretical understanding of, and hands-on experience with, state-of-the-art equipment used in quantitative fluorescence microscopy, including: wide-field fluorescence microscopy, laser scanning and spinning disk confocal microscopy, total internal fluorescence microscopy (TIRF), super-resolution methods (structured illumination, STED, STORM and PALM) and digital image processing and analysis. Students will learn how to design and implement a wide range of imaging experiments using these techniques. Students will use the techniques to address specific quantitative questions and then discuss the results as a group, learning to troubleshoot the common problems that occur in the course of a quantitative imaging experiment. Among the lectures presented are: quantitative microscopy basics, transmitted light microscopy, image segmentation, image analysis, CCD & sCMOS cameras, confocal microscopy, multi-photon microscopy, deconvolution, TIRF, imaging ratio-metric “biosensors” (including FRET), light sheet microscopy and super-resolution techniques. Students will also learn guidelines for choosing fluorescent proteins, and work with live samples requiring environmental control. In addition to the course instructors listed above, lecturers for 2015 include Nathan Shaner (The Scintillon Institute), Talley Lambert (Harvard Medical School), Dylan Burnette (Vanderbilt), and Pavel Tomancak (Max Planck Institute of Molecular Cell Biology & Genetics).
- Organization: Cold Spring Harbor Laboratory
Wed, Apr 08, 2015 - Tue, Apr 21, 2015
CSHL Campus
Cold Spring Harbor, United States
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