High-throughput methods for Protein Production and Crystallization
EMBO Practical Course - Harwell, United Kingdom
Structural proteomic projects have been established world-wide to carry out high throughput structure determination on a genome-wide scale. Crucial to this effort has been the development of HTP technologies for protein production, protein crystallization and improved methods for structure solution by X-ray crystallography. The overall aim of the course will be to review the state-of-the-art in HTP structural biology with an emphasis on methods to study complex targets, including membrane proteins.
Practical training will be provided in:
Structural bioinformatics (lecture & workshop)
High throughput cloning & expression in E. coli and mammalian cells (lectures & practicals)
Protein purification and biophysical characterisation (lectures, practicals and demonstrations).
High throughput protein crystallization (lecture & practicals).
High throughput data collection at synchrotrons (lectures & workshop).
(Courtesy of Microscopic views of different protein crystal morphologies, via bayertechnology.com)
High throughput Protein Production and ... - Events - EMBO
Wed, May 15, 2013 - Thu, May 23, 2013
Research Complex at Harwell, R92 Rutherford Appleton Laboratory
Harwell Oxford, Oxfordshire OX11 OFA, UK
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