Practical Laboratory Training in Molecular Biology & Protein Expression - University of Warwick
The vast majority of this two week intensive course consists of hands-on laboratory work, providing the opportunity to learn vital and fundamental laboratory skills. An excellent theoretical background to the techniques is provided through accompanying discussion. The course will provide the opportunity to demonstrate your skill in the laboratory to employers through your own individual lab book write-up. The course will also give an insight into state-of-the-art methodologies used in a broad range of topics.
This course is deisgned for any one wanting a basic, hands-on introduction to practical biochemistry and molecular biology techniques including new graduates, health professionals and pharmaceutical industry personnel.
It is particularly suitable for Biotechnology, Bioprocessing and Business Management MSc students who need laboratory experience.
Purification and analysis of plasmid DNA. To include;
Mini-preps
Agarose gel electrophoresis
Restriction digests
Making plates
PCR mutagenesis techniques. To include;
Preparation of DNA for sequencing
Polymerase Chain Reaction
Primer design
Cloning into an E.coli expression vector. To include;
sequencing
analysis of sequencing data by computer
Overexpression of protein in E.coli. To include;
Growing a culture
Making a buffer
Lactate dehydrogenase (LDH) expression
Purification of protein using affinity chromatography and SDS gel electrophoresis. To include;
Use of sonicator or chemical cell lysis
Running a column
SDS gel electrophoresis
Protein analysis. To include;
SDS gel interpretation
spectrophotometric enzyme assays
kinetic analysis
(Courtesy of http://www.warwick.ac.uk/go/labskills)
Mon, Sep 03, 2012 - Fri, Sep 14, 2012
School of Life Sciences, University of Warwick
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