Spotlight on Science Meetings, Conferences and Events brings you information on the following event: T Cell Flow Cytometry: Analyzing Antigen, Specific T Cells Extra, and Intracellularly
This course provides hands-on experience for detecting and analyzing antigen-specific and non-specific human T cell subsets. Participants will be introduced to immunomagnetic pre-enrichment as a simple method enabling the reliable detection and analysis of rare cell types.
Two different technical approaches will be shown and performed in the lab:
1.Enrichment of antigen-specific CD4+ T cells by using an activation marker in combination with intracellular cytokine staining.
2.Analysis and enrichment of viable cytokine producing antigen-specific T cells using Cytokine Secretion Assay technology.
T cells play an essential role in the immune system, as they are responsible for initiation and regulation of immune responses to pathogens. They are a heterogeneous cell population, comprising a multitude of subpopulations with distinct functions and phenotypes. Flow cytometry is a widely used method for cell analysis and is often used to elucidate the functions and roles of T cells in health and disease. However, the detection of antigen-specific T cells, for example, is hampered by their very low frequency.
The training covers all subjects from a profound introduction via hands-on training through to comprehensive troubleshooting.
MACS Academy
San Diego, CA
http://www.miltenyibiotec.com/en/support/macs-academy/hands-on.aspx
Thursday May 4, 2017 to Friday, May 5, 2017