Course - Cold Spring Harbor, NY, United States
This intensive laboratory and lecture course will focus on cutting-edge proteomic approaches and technologies.Students will gain practical experience purifying and identifying protein complexes and posttranslational modifications. In a section focused on quantitative whole proteome analyses or top-down proteomics, students will gain hands-on experience using two-dimensional gel electrophoresis and mass spectrometry analysis. Students will use differential in-gel electrophoresis (DIGE) for gel-based protein quantification. Differentially expressed proteins will be recognized by statistical methods using advanced gel analysis software and identified using MALDI mass spectrometry. For shotgun proteomic analysis sections or bottom-up proteomics, students will use label-free and covalent isotopic-labeling quantitative approaches to differentially profile changes in protein complexes and whole proteomes.
Organization: Cold Spring Harbor Laboratory
Tue, Jul 15, 2014 - Mon, Jul 28, 2014
CSHL Campus
Cold Spring Harbor, United States
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