Spotlight on Science Meetings, Conferences and Events brings you information on the following event: Analytical and Quantitative Light Microscopy
This course provides a systematic and in-depth examination of the theory of image formation and application of video and digital methods for exploring subtle interactions between light and the specimen. This course emphasizes the quantitative issues that are critical to the proper interpretation of images obtained with modern wide-field and confocal microscopes.
Laboratory exercises, demonstrations, and discussions include:
- Geometrical and physical optics of microscope image formation including Abbe’s theory of the microscope and Fourier optics
- Interaction of light and matter
- Phase contrast polarization and interference microscopy for the nondestructive analysis of molecular and fine-structural organization in living cells
- Fluorescence microscopy, quantification of fluorescence, and GFP
- Principles and application of digital video imaging, recording, analysis, and display
- Digital image processing and quantitative digital image deconvolution
- Ratiometric measurement of intracellular ion concentrations
- Confocal microscopy
- New advances in light microscopy such as FRET, FLIM, TIRF, and patterned illumination.
Lectures are followed by small group laboratory sessions and demonstrations. As a result, students will have opportunities for extensive hands-on experience with state-of-the-art optical, electronic, and digital imaging equipment guided by an experienced staff from universities and industry.
The program is designed primarily for university faculty, professional researchers, postdoctoral fellows, and advanced graduate students in the life sciences who wish to expand their experience in microscopy and to understand the quantitative issues associated with analysis of data obtained with optical microscopes.
University of Chicago, IL
http://www.mbl.edu/education/courses/analytical-quantitative-light-microscopy/
Wednesday, May 3, 2017 to Friday, May 12, 2017
