Enhanced rare cell analysis – Visualize the invisible
Course - Cologne, Germany
Flow cytometry is a widely used method for cell analysis. It allows the acquisition of a large number of cells, resulting in statistically reliable analysis of cell populations. However, when the frequency of target cells is below 0.1%, the data becomes inaccurate. Immunomagnetic cell enrichment before flow cytometric measurement resolves this problem by increasing the frequency of target cells. This simple step allows for the visualization of such rare cell populations. The training covers this approach from introduction to trouble-shooting with theory and extensive hands-on practice in the laboratory.
Course lectures and exercises include:
Introduction to rare cell analysis
Introduction to immunomagnetic cell enrichment
Detetction of circulating endothelial cells (cEC)
Antigen-specific T cell cytometry
Practical laboratory parts include:
Preparation of blood samples
Immunofluorescent labeling of cells (surface and intracellular)
Immunomagnetic labeling and magnetic cell separation
In vitro stimulation of leukocytes
Flow cytometric detection and characterization of rare cell populations
Organization: Miltenyi Biotec
(Courtesy of Flow Cytometry Cell Cycle Analysis, via labmonsters.com)
Enhanced rare cell analysis – Visualize the invisible
Wednesday, October 30, 2013 - Thursday, October 31, 2013 (9:00 AM - 4:00 PM)
MACS Academy, Miltenyi Biotec GmbH, Friedrich-Ebert-Str. 68, Bergisch Gladbach/Cologne, Germany
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