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Enhanced rare cell analysis – Visualize the invisible: Laboratory/lecture Course

Posted on Sep 1, 2013 5:30:00 AM

Enhanced rare cell analysis – Visualize the invisible

Course - Cologne, Germany

Flow cytometry is a widely used method for cell analysis. It allows the acquisition of a large number of cells, resulting in statistically reliable analysis of cell populations. However, when the frequency of target cells is below 0.1%, the data becomes inaccurate. Immunomagnetic cell enrichment before flow cytometric measurement resolves this problem by increasing the frequency of target cells. This simple step allows for the visualization of such rare cell populations. The training covers this approach from introduction to trouble-shooting with theory and extensive hands-on practice in the laboratory.

Course lectures and exercises include:

Introduction to rare cell analysis

Introduction to immunomagnetic cell enrichment

Detetction of circulating endothelial cells (cEC)  

Antigen-specific T cell cytometry

Practical laboratory parts include:

Preparation of blood samples

Immunofluorescent labeling of cells (surface and intracellular)

Immunomagnetic labeling and magnetic cell separation

In vitro stimulation of leukocytes

Flow cytometric detection and characterization of rare cell populations

Organization: Miltenyi Biotec

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(Courtesy of Flow Cytometry Cell Cycle Analysis, via labmonsters.com)

Enhanced rare cell analysis – Visualize the invisible

Wednesday, October 30, 2013 - Thursday, October 31, 2013 (9:00 AM - 4:00 PM)

MACS Academy, Miltenyi Biotec GmbH, Friedrich-Ebert-Str. 68, Bergisch Gladbach/Cologne, Germany

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Tags: 2013, cell biology, flow cytometry, germany, Cologne, October, Life Sciences, laboratory

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