Science Researcher Update

2015 Yeast Genetics & Genomics Course

Written by Stela BCI | Jun 4, 2015 9:24:00 AM
 
Course - Cold Spring Harbor, NY, United States
 
The Yeast Genetics & Genomics course is a modern, state of the art laboratory course designed to teach students the full repertoire of genetic approaches needed to dissect complex problems in the yeast Saccharomyces cerevisiae. Combinations of classical and modern genetic approaches are emphasized, including the isolation and characterization of mutants, tetrad analysis, complementation, and mitotic recombination. Students will learn array-based methods, next generation sequencing, and genome-based methods of analysis facilitated by the yeast genome sequence, the deletion collection, and other genomic resources available to the community. Molecular genetic techniques, including yeast transformation, gene replacement by PCR, construction and analysis of gene fusions, and generation of mutations, will also be emphasized.
Students will use classical approaches and modern whole genome sequencing to gain experience in identifying and interpreting various kinds of genetic interactions including suppression and synthetic lethality (including SGA). Students will be immersed in yeast genomics and will perform and interpret experiments using DNA arrays, whole genome sequencing, and multiplexed DNA barcode sequencing. Students will gain first-hand experience in modern cytological approaches such as epitope tagging and imaging yeast cells using indirect immunofluorescence, GFP-protein fusions and a variety of fluorescent indicators for different subcellular organelles. Lectures on fundamental aspects of yeast genetics will be presented along with seminars given by prominent experts in the field on topics of current interest.
Organization: Cold Spring Harbor Laboratory
Yeast Genetics & Genomics
Tue, Jul 21, 2015 - Mon, Aug 10, 2015
CSHL Campus
Cold Spring Harbor, United States
 
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