Course - Cold Spring Harbor, NY, United States
This intensive lecture and laboratory course is designed for biologists interested in using mouse models to study mammalian development, stem cells and cancer.Lecture portion of the course, taught by leaders in the field, provides the conceptual basis for contemporary research in embryogenesis, organogenesis, embryonic, adult and induced pluripotent stem cells and cancer biology. Laboratory portion of the course provides extensive hands-on introduction to engineering of mouse models, stem cell technologies and phenotyping.
The experimental techniques include genome editing by CRISPR/Cas9, pronuclear microinjection, isolation, culture and manipulation of pre- and post-implantation embryos, embryo transfer, embryo electroporation and roller culture, embryo and sperm freezing, genetic manipulation of embryonic stem cells, production of chimeras, the generation and differentation of embryonic stem cells as well as induced pluripotent stem cells, isolation of mouse embryonic fibroblasts, cell scratch assay, focus formation assay, teratoma formation assay, vibratome and cryosectioning, in situ hybridization, immunostaining, vascular injections, skeletal preparation, organ explant culture, confocal imaging, and live time-lapse microscopy of early embryos, explanted tissues, and tumors in vivo.
Organization: Cold Spring Harbor Laboratory 2014
Cold Spring Harbor Laboratory 2014 Course - Mouse Development, Stem Cells & Cancer
Wed, Jun 04, 2014 - Tue, Jun 24, 2014
CSHL Campus
Cold Spring Harbor, United States
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