EMBL Course: Next Generation Sequencing: Whole Genome Sequencing Library Preparation

 

Course - Heidelberg, Germany

 

The field of DNA sequencing has evolved rapidly in the last years and the rise of next generation sequencing (NGS) has caused a dramatic decrease in sequencing costs. These new technologies have fostered the development of a plethora of new applications and enabled new experiments, study designs, and fields of research. Therefore the numbers of NGS users are constantly growing and there is an increasing demand for basic trainings which we want to address with this course.

The goal of this course is to teach the fundamentals of NGS library preparation and validation using the Illumina TruSeq DNA PCR-free sample preparation kit. We will start with an introduction into Illumina NGS technology and the library preparation workflow. The main part will be the preparation of libraries from genomic DNA, with a focus on the critical steps and potential pitfalls of the protocol. Finally we will quantify and quality control the prepared libraries, before discussing solutions for whole genome sequencing data analysis.

During this course we will focus on the following aspects:

Best practices and tips and tricks for TruSeq DNA PCR-free library preparation
Best practices of sequencing library validation
Introduction to whole genome sequencing data analysis