Science Researcher Update

Principles, parameters & applications of PCR - Biomedical Laboratory Course

Written by BCI Staff | Jun 14, 2013 10:05:00 AM

Principles, parameters and applications of PCR

Course - Breda, Noord-Brabant, The Netherlands

Aim of the course:  

This short course aims at training the participant in the basic principles and applications of the Polymerase Chain Reaction (PCR) and real-time PCR. The program consists of a series lectures and experiments covering all basic PCR topics like efficiency, fidelity and specificity of the PCR reaction.

Participants:  

This course is developed as an introduction to the PCR and especiall suitable as a basic introduction to our advanced course on Real-time PCR in molecular diagnostics (2548). Basic knowledge of molecular biology in general is desirable.

Course content:

Theory:

Principles of DNA amplification technologies; The biochemical and physical aspects of the PCR

Principles, parameters, and design of (real-time) PCR primers

Principles and parameters of detection of PCR products

Principles of Real-time PCR; detection formats, assay formats

PCR contamination prevention

Practicals:

Real-time PCR dilution series and melting curve analysis:

Optimization and determination of the sensitivity of real-time PCR with SybrGreen

Optimization and determination of the sensitivity of real-time PCR with Hybridisation probes

(Photo courtesy of PCR tubes, each tube contains a 1000uL (1mL) reaction, via en.wikipedia.org)

For more information, please visit: http://www.alsavans.nl/avans-training-2545.htm

October 22, 2013 - October 24, 2013  (9.00 - 18.00)

Avans University, Breda, Noord-Brabant, The Netherlands

Contact: Jeanie (j.blom5@avans.nl)

Phone: 076 525 0575

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