RNA-seq Bioinformatics: A Practical Introduction
Workshop - Leipzig, Germany
The purpose of this workshop is to get a deeper understanding in High-Throughput Sequencing (HTS) with a special focus on bioinformatics issues. Advantages and disadvantages of current sequencing machines and their implications on data analysis will be discovered. The participants will be trained on understanding their own HTS data, finding potential problems/errors and finally start writing their own pipelines. In the course we will use a real-life RNA-seq dataset from the current market leader Illumina.
All analyses will be performed using cloud services. By saving their final cloud-images, the participants will be able to reuse all tools/pipelines and to continue their analyses after the workshop(platform independently: Windows, Mac OS, Linux).
By the end of this workshop the participants will:
be familiar with the sequencing methods from various instruments:
– Illumina HiSeq 2000
– Roche 454
– Ion Torrent
be aware of the different error sources of these machines
understand common data formats and standards
know relevant tools for HTS data processing
automate tasks with shell scripting to create reusable data pipelines
perform basic analyses (gene quantification, differential expression)
plot and visualize results
be able to reuse all analyses via the employed cloud environment
(Courtesy of RNA-seq Bioinformatics: A Practical Introduction logo, via ecseq.com)
RNA-seq Bioinformatics: A Practical Introduction - ecSeq ...
Mon, May 13, 2013 - Tue, May 14, 2013
UFZ Conference Venue, Helmholtz Centre for Environmental Research, Permoserstrasse 15, Leipzig, Germany
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