Introduction to Molecular Biology Techniques Biotech Course

Molecular biology pertains to the study of living systems at the molecular level, especially DNA and RNA, and provides a background appropriate for further work in the rapidly expanding areas of genomics, cell biology, biotechnology, microbiology, diagnostics, and therapeutics. This course will focus on selected aspects of molecular biology that provide the non-specialist with the principles for understanding the structure and functional relationships of molecular biology techniques including DNA manipulation, sequencing, cloning, subcloning, library construction, screening, RNA isolation and characterization, analysis of expression, cDNA synthesis (RT-PCR) and analysis, microarrays and gene chips, and Real-Time-PCR. Multiple modern day molecular biology techniques in the biotechnology and pharmaceutical industries will be presented and several examples of molecular applications will be highlighted.

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