ABCAM Webinar on Life Sciences: Major Scientific Discoveries

ChIP is a very powerful technique that allows the localization of proteins on DNA regions in the genome. The principle is simple; the selective enrichment of a chromatin fraction containing a specific antigen. Antibodies that recognize a protein or protein modification are used to determine the relative abundance in the genome in vivo. The ChIP technique can be used in any area of research to further elucidate gene function and regulation in their native state.

Webinar Topics:
• Introduction to chromatin, ChIP (Chromatin IP) and usages of the method
• Overview of genomic approaches to map in-vivo chromatin structure (ChIP-seq and ChIP-chip)
• Detailed description of genome-wide mapping of chromatin by ChIP-seq and ChIP-chip – e.g. analysis methods, validations, sample requirements, reproducibility, etc.
• Usage of visualization tools such as IGV and UCSC Genome Browser.
• Major scientific discoveries stemming from charting of in-vivo chromatin maps – chromatin states, enhancer mapping, organization of the chromatin regulators, dynamics of transcription factors binding

http://www.abcam.com/