Course - Cold Spring Harbor, NY, US
The Eukaryotic Gene Expression Course is designed for students, postdocs, and principal investigators who have recently ventured into the exciting area of gene regulation. The course will focus on state-of-the-art strategies and techniques employed in the field. Emphasis will be placed both on in vitro and in vivo protein-DNA interactions and on novel methodologies to study gene regulation. Students will make nuclear extracts, perform in vitro transcription reactions and measure RNA levels using primer extension. Characterizations of the DNA-binding properties of site-specific transcription factors will be carried out using electrophoretic mobility shift and DNase I footprinting assays. In addition, students will learn techniques for the assembly and analysis of chromatin in vitro. This will include transcription assays, chromatin footprinting and chromatin remodeling assays.
Experience with basic recombinant DNA techniques is a prerequisite for admission to this course. Lectures by the instructors will cover the current status of the gene expression field, theoretical aspects of the methodology, and broader issues regarding strategies for investigating the regulation of gene expression in eukaryotes. Guest lecturers will discuss contemporary problems in eukaryotic gene regulation and technical approaches to their solution.
(Photo courtesy of http://www.cshl.edu/meetings)
24th July - 13th August 2012
Cold Spring Harbor Laboratory Meetings & Courses Program, 1 Bungtown Road, Cold Spring Harbor, NY 11724
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