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T cell flow cytometry – analyzing antigen-specific T cells extra - & intracellulary

Posted on May 13, 2015 11:31:00 AM

 
Course - Bergisch Gladbach, Germany
 
T cells play an essential role in the immune system, as they are responsible for initiation and regulation of immune responses to pathogens. T cells are a heterogeneous cell population, comprising a multitude of subpopulations with distinct functions and phenotypes. Flow cytometry is a widely used method for cell analysis and is often used to elucidate the functions and roles of T cells in health and disease. However, the detection of antigen-specific T cells, for example, is hampered by their very low frequency.
This course provides hands-on experience for detecting and analyzing antigen-specific and non-specific human T cell subsets. Participants will be introduced to immunomagnetic pre-enrichment as a simple method enabling the reliable detection and analysis of rare cell types. By combining this method with intracellular cytokine staining, antigen-specific T cell subsets can be analyzed in detail. Furthermore, this course conveys both theoretical aspects and extensive practical experience of multicolor analysis of numerous T cell subsets from human blood.
The training covers all subjects from a profound introduction via hands-on training through to comprehensive troubleshooting.
Wed, Jun 24, 2015 - Thu, Jun 25, 2015
MACS Academy
Friedrich-Ebert-Str. 68, Bergisch Gladbach, Germany
 
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Tags: June, flow cytometry, Immunology, Immune System, germany, 2015, research course, Life Sciences

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