Course - Hinxton, Cambridge, United Kingdom
This laboratory-based training course will provide a comprehensive overview and practical laboratory experience of the genetic manipulation of mammalian stem cells.Focusing on mouse ES and human iPS cells, genome informatics, recombineering, CRISPR designer nucleases, transposon technology and gene targeting will be covered in interactive demonstrations, discussions and lectures by international experts. Laboratory work includes the culture and transfection of mouse ES and human iPS cells, design and construction of gene targeting vectors, and the use of PiggyBac transposons and designer nucleases (CRISPRs) for advanced genome engineering and genetic screens. Participants will be trained in the informatics and practical use of public IKMC (International Knockout Mouse Consortium) mouse knockout resources, including the production of mutant mice.Emphasis is placed on the planning/design and successful experimental execution of gene targeting projects and participants should gain the skills necessary to design, construct and target their own genes of interest.
Organization: Wellcome Trust Advanced Courses
16 February 2015 - 28 February 2015
Wellcome Trust Genome Campus,
Hinxton, Cambridge CB10 1SA, UK
Contact: Wellcome Trust Advanced Courses (email@example.com)
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