Spotlight on Science Meetings, Conferences and Events brings you information on the following event: Chromatin, Epigenetics and Gene Expression
The Chromatin, Epigenetics and Gene Expression course is designed for students, postdocs, and principal investigators who have recently ventured into the exciting area of gene regulation. Emphasis will be placed on exposing students to a broad array of methodologies to study gene regulation, chromatin structure and dynamics, including both state-of-the-art and well-developed methods.
Students will perform widely used techniques such as chromatin immunoprecipitation (ChIP) coupled with sequencing (ChIP-seq), reporter assays of enhancer activity, and RNA analysis. They will apply a basic pipeline to analyze sequencing results and will discuss current informatics strategies. Students will isolate transcription factor complexes and assess their activity in functional assays, and will knock-down specific factors using RNAi and evaluate the effects on gene expression.
This course will provide the basic concepts behind different methods to analyze the chromatin architecture of the genome. Students will perform Chromosome Conformation Capture (3C) experiments, together with other approaches aimed to interrogate the 3D organization of genomes. Moreover, we will discuss the computational methods required to analyze these data.
Students will learn how to assemble recombinant chromatin and use biophysical methods such as FRET to assay the activity of chromatin remodeling enzymes. They will also learn principles of enzyme kinetics and will apply these to quantify the remodeling reactions.
Cold Spring Harbor Laborator
Cold Spring Harbor, NY
Phone: (516) 367-8346
Tuesday, July 25, 2017 to Monday, August 14, 2017