UW Genomics Researchers Find Genes Linked to Autism in the Exome
A team of Genomics Researchers at the University of Washington, Seattle have recently uncovered several sporadic gene mutations in the protein producing areas of the genome, called the exome, that may be linked to autism spectrum disorders. The scientists used the latest molecular biology techniques as well as parallel sequencing to simultaneously examine the exomes of several children with a certain form of autism.
The research was led by Brian O’Roak, Evan Eichler, and Jay Shendure of the UW Department of Genome Sciences. It was primarily funded by the American Recovery and Reinvestment Act as well as the NIH.
(left: Brian O'Roak, seated: Evan Eichler, right: Jay Shendure, Photo Credit: Clare McLean)
The UW genomics researchers used a technique called trio-based exome sequencing to compare and examine the exomes of 20 families. The method involves simultaneously sequencing the genomes of a child and both their parents in order to look for new mutations in the children. The researchers found 21 new sporadic mutations in the exomes of the children, of which 11 had an effect on protein production.
In particular, in four of the studied families the researchers found sporadic mutations that were likely linked to autism. The children in these families showed the most severe symptoms of the disorder. These findings suggest that sporadic mutations may be linked with the underlying biology and severity of autism, particularly in families with no history of the disorder.
(image of color-coded data generated during exome sequencing courtesy of UWnews)
The reasons for sporadic mutation is still unknown, but this UW genomics study has identified several risk factors. The scientists were able to trace the origin of the mutations back to the father's genome and, in one case, to the mother's genome. This is consistent with population studies that have shown that children with older parents, particularly older fathers, are more at risk for autism. Eichler suggested that this trend may be caused by the increased likelihood of certain genetic mutations during sperm formation that comes with aging.
The researchers hope that the data gathered in this study will help with understanding the biology and causes of autism while suggesting new research directions and possible treatments.
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