Course - Hinxton, Cambridge, United Kingdom
This laboratory-based training course will provide a comprehensive overview and practical laboratory experience of the genetic manipulation of mammalian stem cells. Focusing on mouse ES and human iPS cells, recent advances in genome informatics, recombineering, custom nucleases (CRISPRs/TALENs), transposon technology and conditional gene targeting will be covered in lectures and through interactive demonstrations. Laboratory work includes the culture and transfection of ES cells, design and construction of gene targeting vectors by BAC recombineering, and the use of PiggyBac transposons and designer nucleases (CRISPRs/TALENs) for advanced genome engineering. Participants will be trained in the informatics and practical use of public gene targeting resources, including the production of mutant mice from the IKMC (International Knockout Mouse Consortium) resource. Emphasis is placed on the planning/design and successful experimental execution of gene targeting projects.
(Courtesy of Adult mammalian stem cells, via nature.com)
March 10, 2014 - March 22, 2014
Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom
Contact: Wellcome Trust Advanced Courses (firstname.lastname@example.org)
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