Science Researcher Update: Spotlight on Meetings, Conferences, and Events

Principles, parameters & applications of PCR - Biomedical Laboratory Course

Posted on Jun 14, 2013 3:05:00 AM

Principles, parameters and applications of PCR

Course - Breda, Noord-Brabant, The Netherlands

Aim of the course:  

This short course aims at training the participant in the basic principles and applications of the Polymerase Chain Reaction (PCR) and real-time PCR. The program consists of a series lectures and experiments covering all basic PCR topics like efficiency, fidelity and specificity of the PCR reaction.

Participants:  

This course is developed as an introduction to the PCR and especiall suitable as a basic introduction to our advanced course on Real-time PCR in molecular diagnostics (2548). Basic knowledge of molecular biology in general is desirable.

Course content:

Theory:

Principles of DNA amplification technologies; The biochemical and physical aspects of the PCR

Principles, parameters, and design of (real-time) PCR primers

Principles and parameters of detection of PCR products

Principles of Real-time PCR; detection formats, assay formats

PCR contamination prevention

Practicals:

Real-time PCR dilution series and melting curve analysis:

Optimization and determination of the sensitivity of real-time PCR with SybrGreen

Optimization and determination of the sensitivity of real-time PCR with Hybridisation probes

untitled

(Photo courtesy of PCR tubes, each tube contains a 1000uL (1mL) reaction, via en.wikipedia.org)

For more information, please visit: http://www.alsavans.nl/avans-training-2545.htm

October 22, 2013 - October 24, 2013  (9.00 - 18.00)

Avans University, Breda, Noord-Brabant, The Netherlands

Contact: Jeanie (j.blom5@avans.nl)

Phone: 076 525 0575

 If you'd like to ask a question or post a comment about this talk please do so below.

This seminar posting is brought to you by Biotechnology Calendar, Inc. providing access to research information and research tools for nearly 20 years. Visit our Science Market Update Blog for current science funding and market information or see our schedule of upcoming science research laboratory product shows.

Tags: 2013, biomedical research, Molecular Biology, Netherlands, October, Breda, life science research, Life Sciences, Genetics, laboratory, research laboratories

This meeting information is brought to you by Biotechnology Calendar, Inc

We connect researchers with the tools they need to succeed. 

Get 2020 Schedule

sell more lab supplies