Course - The Laboratory Citadel Hill, Plymouth, United Kingdom
There will be 2–3 lectures each day throughout. Lectures will cover topics from basic optics, detectors and light sources through to photochemistry involved in the development of novel fluorescent probes and the application of advanced super-resolution optical microscopy techniques. The first 3 days will be spent setting up microscopes for brightfield and epifluorescence microscopy, including polarisation and interference contrast methods. The final 6 days will be organised in rotations around the 9 experiments listed below. The experiments will be in the large classroom at the MBA and in rooms nearby. Use will be made of marine specimens where possible and student may have an opportunity to trawl for benthos and plankton, depending on weather and availability of the MBA research vessel.
Developmental Biology
Experiments with confocal imaging
CCD imaging and deconvolution, microinjection, genetically encoded probes, gene gun.
Cell Physiology
Confocal imaging with electrophysiology
CCD imaging with electrophysiology
Photolysis and photometry
Total internal reflection fluorescence imaging
Laser traps
Marine bioluminescence
Special demonstrations and hands-on exercises
Multi-photon and confocal imaging
Quantitative interference microscopy
Use of an optical bench for basic experiments on scanning and apertures
Spectroscopy and filters
Abbe’s experiments on the role of diffraction in microscope image formation with absorption and phase objects.
EMBO Practical Course: 'Advanced Optical Microscopy'
Wed, Apr 03, 2013 - Sat, Apr 13, 2013
The Marine Biological Association, The Laboratory Citadel Hill
Plymouth PL1 2PB U.K.
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